Molecular cloning of an immunodominant antigen of Onchocerca volvulus.
نویسندگان
چکیده
منابع مشابه
Molecular cloning of an immunodominant antigen of Onchocerca volvulus
We have cloned and characterized the gene for an immunodominant antigen of O. volvulus that is recognized by the sera of 96% of patients with onchocerciasis. Its 1.2-kb mRNA constitutes 0.3% of adult worm poly(A)+ RNA and its cDNA sequence reveals that it is not a highly conserved structural protein such as actin or tubulin. Similar but not identical genes occur in the genomes of related filari...
متن کاملMolecular cloning of adenylate kinase from the human filarial parasite Onchocerca volvulus
Adenylate kinases (ADK) are ubiquitous enzymes that contribute to the homeostasis of adeninenucleotides in living cells. In this study, the cloning of a cDNA encoding an adenylate kinase from the filariaOnchocerca volvulus has been described. Using PCR technique, a 281 bp cDNA fragment encoding part ofan adenylate kinase was isolated from an O. volvulus cDNA library. Use of this fragment as a p...
متن کاملCharacterization of an immunodominant Onchocerca volvulus antigen with patient sera and a monoclonal antibody
Adult Onchocerca voluvlus and infective larvae, but not microfilariae contain an immunodominant antigen (33,000 and 21,000 Mr in females, 39,000, 33,000, and 21,000 Mr in males, 133,000 Mr in infective larvae) which is recognized by an Onchocerca-specific mAb. The component is part of the reproductive organs and muscles. 96.2% of onchocerciasis sera contained antibodies detectable by immunoblot...
متن کاملmolecular cloning of adenylate kinase from the human filarial parasite onchocerca volvulus
adenylate kinases (adk) are ubiquitous enzymes that contribute to the homeostasis of adeninenucleotides in living cells. in this study, the cloning of a cdna encoding an adenylate kinase from the filariaonchocerca volvulus has been described. using pcr technique, a 281 bp cdna fragment encoding part ofan adenylate kinase was isolated from an o. volvulus cdna library. use of this fragment as a p...
متن کاملMolecular characterization and expression of Onchocerca volvulus glutathione reductase.
Glutathione metabolism represents a potential target for anti-parasite drug design. The central role of glutathione reductase (GR) in maintenance of the thiol redox state and in anti-oxidative defence has to be evaluated in more detail in order to establish the essential function of this enzyme for the survival of the filarial parasite Onchocerca volvulus. The O. volvulus GR (OvGR) gene was clo...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1988
ISSN: 0022-1007,1540-9538
DOI: 10.1084/jem.168.3.1199